Biological Crystallization

Crystallization of Biological Macromolecules

All of the macromolecules are polymers consist of amino acids, ribonucleotides and deoxyribonucleotides, sugars of various sorts, fatty acids, etc. Crystallization of macromolecules is complex process based on finding individual conditions and parameters leading to formation of crystal. Experience and reproducibility are guides in making crystallization experiments. All of the macromolecules ar...

Automation in biological crystallization.

Crystallization remains the bottleneck in the crystallographic process leading from a gene to a three-dimensional model of the encoded protein or RNA. Automation of the individual steps of a crystallization experiment, from the preparation of crystallization cocktails for initial or optimization screens to the imaging of the experiments, has been the response to address this issue. Today, large...

Development of Crystallization Strategies Using the Biological Macromolecule Crystallization Database

The NIST/NASA/CARB Biological Macromolecule studies requires not only finding suitable chemical agents Crystallization Database (BMCD) contains crystal data that induce and sustain crystal growth but also that those and crystallization conditions for biological parameters such as protein concentration, ionic strength, macromolecules abstracted from the literature. Each temperature, pH, etc., be...

Biological Nano-crystallization ▶Macromolecular Crystallization Using Nano- volumes Biological Photonic Structures

Induction of Rules for Biological Macromolecule Crystallization

X-ray crystallography is the method of choice for determlning the 3-D structure of large macromolecules at a high enough resolution. The rate limiting step in structure determination is the crystallization itself. It takes anywhere between a few weeks to several years to obtain macromolecuiar crystals that yield good diffraction patterns. The theory of forces that promote and maintain crystal g...